Journal: Marine Drugs

Article Title: Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

doi: 10.3390/md13042063

Figure Lengend Snippet: Activated partial thromboplastin (APTT) and IC 50 of the sulfated fucans for thrombin or factor Xa inhibition in the presence of antithrombin or heparin cofactor II.

Article Snippet: Biophen Heparin Anti-IIa kits, Biophen Antithrombin 2.5 kits, Human HCII, ATIII, thrombin, thrombin chromogenic substrate CS-01(38) and factor Xa chromogenic substrate SXa-11 were all from Hyphen Biomed (Paris, France).

Techniques: Inhibition

Journal: Marine Drugs

Article Title: Structural Analysis and Anticoagulant Activities of the Novel Sulfated Fucan Possessing a Regular Well-Defined Repeating Unit from Sea Cucumber

doi: 10.3390/md13042063

Figure Lengend Snippet: Inhibitory effects of the sulfated fucans, heparin, LMWH and DS on factor Xa in the presence of antithrombin. ( A ) Shows the time course of Xa inhibition. Mixed samples of 30 μL of polysaccharides (625 ng/mL) and 30 μL of 1 IU/mL AT were incubated at 37 °C for 2 min, and 30 μL of 8 μg/mL bovine Xa was then added. After incubation for 1 min, 30 μL of 1.20 mM CS-11(65) was added, the residual Xa activity was recorded by absorbance at 405 nm; ( B ) Shows the dependence on the sulfated polysaccharide concentration for Xa inactivation in the presence of AT. The reaction mixtures were as described in ( A ), except that different concentrations of sulfated polysaccharides were used. Results are shown as means of duplicates. See for IC 50 values.

Article Snippet: Biophen Heparin Anti-IIa kits, Biophen Antithrombin 2.5 kits, Human HCII, ATIII, thrombin, thrombin chromogenic substrate CS-01(38) and factor Xa chromogenic substrate SXa-11 were all from Hyphen Biomed (Paris, France).

Techniques: Inhibition, Incubation, Activity Assay, Concentration Assay